英语翻译3.免疫组织化学收集临床结肠癌患者的组织标本及完整的临床病例资料,免疫组织化学SP法检测35例结肠癌患者的正常食

3. 免疫组织化学
收集临床结肠癌患者的组织标本及完整的临床病例资料,免疫组织化学SP法检测35例结肠癌患者的正常食管组织,癌旁组织和癌组织中p27的表达,并根据临床资料进行统计分析结肠癌中p27的蛋白表达与临床病理特征间的关系及意义.
3. Immunohistochemistry
The tissue samples of clinical colon carcinoma patients and full clinical data of their cases were collected; the normal esophageal tissues, tumor-adjacent tissues and p27 expression in the carcinoma tissues of 35 patients of colon carcinoma were examined by immunohistochemical SP method, and based on the clinical data, statistic analysis was conducted on the relationship and significance between protein expression of p27 in colon carcinoma and the clinicopathological features.
4. 图像处理及统计学分析
Western blotting和RT-PCR结果用IPP6.0进行灰度分析,实验重复三次,应用SPSS13.0软件采用单因素方差分析（one-way ANOVA）,P