哈_焊太阳
幼苗
共回答了15个问题采纳率:80% 举报
The gene treatment main way has two,namely ex vivo and in vivo way.In the vivo way needs the carrier directly to induct the extraneoussource gene in vivo.This kind of carrier may be the virus or the non-virus.The commonly used viral carrier has the reverse to record thevirus,the adenovirus and so on.The reverse records the virus isthrough mutually affects with the host cell surface special acceptorinfects the host cell.Enters the host cell afterwards 胞浆 center,includes the reverse with the viral pellet in to record the enzyme tostart to reverse records the viral gene group RNA,after the synthesisvirus gene group's linear DNA copy,viral DNA conformity to host genegroup in.The reverse records the viral gene group ingredient,theextraneous source gene and the mark gene which the viral carrierneeds.Lies between leads the gene shift the general processapproximately to contain six step:The clone goal gene,inducts thepacking cell,the screening transformation cell,the elective culture,the infection cell,the analysis gene expression and the treatmentresult.
1年前
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